Plasmid

Part:BBa_K3505009:Design

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-20)


pDGB1 alpha 2 (α2) for Bacterial GoldenBraid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3224
    Illegal XbaI site found at 3251
    Illegal PstI site found at 3263
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3224
    Illegal PstI site found at 3263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3224
    Illegal BamHI site found at 3245
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3224
    Illegal XbaI site found at 3251
    Illegal PstI site found at 3263
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3224
    Illegal XbaI site found at 3251
    Illegal PstI site found at 3263
    Illegal NgoMIV site found at 1291
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3463
    Illegal BsaI.rc site found at 2881